BEAUTY WELLNESS BRAND
Swab:Direct™ Extraction Kit
Dramatically reduce extraction time.
FEATURES
Fast and simple method to extract DNA – Whole process in 5 minutes.
Minimized hands-on time.
Ideal for on-site testing.
Room temperature storage.
01 Swab:Direct™ Extraction Kit
Swab:Direct™ Extraction Kit is designed to perform PCR test from nasopharyngeal and oropharyngeal swab samples without prior DNA/RNA purification process. The buffer includes high concentration of alkaline compounds, so it is optimized for simple extraction of nucleic acids without additional purification process.
02 Comparison of Nucleic acid extraction performance
Comparison tests were performed by extracting nucleic acids from various specimens using Swab:Direct™ Extraction Kit. Extraction assay for each sample was performed 5 times for each extraction method. The table below compares the Ct. values of each test.
1) Human Cell
| No. | Specimen |
Swab:Direct™ Extraction Kit |
Spin Column Method |
Magnetic Bead Method |
|---|---|---|---|---|
| 1 | Nasopharyngeal Swab | 30.58 | 31.29 | 35.52 |
| 2 | Oropharyngeal Swab | 31.43 | 31.79 | 35.66 |
| 3 | Blood Swab | 25.58 | 27.46 | 30.93 |
2) Virus (SARS-CoV-2)
| No. | Specimen |
Swab:Direct™ Extraction Kit |
Spin Column Method |
Magnetic Bead Method |
|---|---|---|---|---|
| 1 | Nasopharyngeal Swab | 30.43 | 31.24 | 35.53 |
| 2 | Oropharyngeal Swab | 30.97 | 31.91 | 35.70 |
| 3 | Blood Swab | 26.32 | 27.75 | 31.24 |
3) Bacteria (E. coli, DH5α)
| No. | Specimen |
Swab:Direct™ Extraction Kit |
Spin Column Method |
Magnetic Bead Method |
|---|---|---|---|---|
| 1 | Nasopharyngeal Swab | 21.30 | 24.62 | 23.71 |
| 2 | Oropharyngeal Swab | 21.44 | 24.91 | 24.22 |
| 3 | Blood Swab | 21.94 | 25.20 | 23.74 |
PROTOCOL
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KIT CONTENTS
| Item | Quantity / Volume | Description |
|---|---|---|
| Swab:Direct™ Extraction Kit | 2 mL x 100 tubes | Extraction Buffer |
ORDERING INFORMATION
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Rapi:Direct™ Lysis Buffer
Makes 'Direct' PCR possible.
FEATURES
Fast and simple method for DNA extraction – whole process in 10 minutes
Minimized hands-on time makes it Ideal for on-site testing.
Various samples including whole blood can be used.
Rapi:Direct™ Lysis Buffer is a single tube DNA extraction system for rapid preparation of DNA from the variety of samples like cell, tissue, blood and serum which enables direct PCR. The formulation of Rapi:Direct™ Lysis Buffer works in two ways – maximizing the lysis efficiency of biological samples and suppressing inhibitory activities of crude lysates, which maintains the integrity of released genomic DNA. With its minimized hands-on time and good yields, Rapi:Direct™ Lysis Buffer is ideal for on-site PCR testing applications.
RESULTS OF PERFORMANCE VERIFICATION TESTS
PCR template volume
Lane M100bp DNA marker.
In this test, 10μl of whole blood sample was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 1μl through 5μl of supernatant from the mixture were used as PCR template.
PCR template volume
Lane M100bp DNA marker.
In this test, 10mg of porcine tissue was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 2μl through 5μl of supernatant from the mixture were used as PCR template.
PROTOCOL
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ORDERING INFORMATION
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Rapi:Prep™ gDNA Extraction Kit
Simple and Efficient Way to Isolate Genomic DNA
FEATURES
Fast and easy process using spin column makes whole extraction process 20 minutes only.
Uses Silica membrane based spin column for efficient extraction.
No phenol and chloroform is included.
Isolates high quality genomic DNA enabling high yield of PCR amplification.
01 Good extraction process makes your test more reliable.
DNA extraction is another important phase to obtain a good result of tests. Genesystem is offering gDNA extraction kit which is well matching with the testing protocols using its ultra-fast PCR platforms.
02 Ideal for fast testing protocol
The Rapi:Prep™ gDNA Extraction Kit provides a fast and simple method to isolate genomic DNA from various animal tissues, cultured cells and bacteria. This kit applies silica-membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. The kit is ready for use and can purify the genomic DNA from a wide variety of animal cell and tissue samples, and the whole process is completed in 20 minutes.
1kb DNA ladder
Kit from Supplier A
Kit from Supplier B
Kit from Supplier C
Kit from Supplier D
Test set-up : Genomic DNA was purified from 20mg of animal tissue using Rapi:Prep™ gDNA Extraction Kit and extraction kits from 4 different competitors respectively. 7μl of the eluate (elution volume: 50 μl) were analyzed on a 1.5% agarose gel.
PROTOCOL
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SPECIFICATION
ORDERING INFORMATION
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Media:Direct™ Extraction Buffer
Dramatically reduce extraction time.
FEATURES
Fast and simple method to extract DNA – Whole process in 5 minutes.
Minimized hands-on time.
Ideal for on-site testing.
Room temperature storage.
01 Media:Direct™ Extraction Buffer
Media:Direct™ Extraction Buffer is designed to perform PCR test with transport media containing viral samples, without prior DNA/RNA purification process. The buffer includes high concentrated alkaline compounds for lysis and elution of sample organisms. This buffer is produced on the basis of the formulation that minimizes PCR inhibiting contents of the samples. This product is easy to use by just mixing the extraction buffer and transport media without additional process for preparing a nucleic acid template to run PCR tests.
02 RESULTS OF PERFORMANCE VERIFICATION TESTS
To comparative experiments between Media:Direct™ Extraction Buffer and others, carried out using GAPDH gene extracted from sample collected by nasopharyngeal and oropharyngeal swab. Each sample was extracted five times per each method. Perform real-time PCR analysis of the extracted nucleic acid from each extraction method using GENECHECKER® UF-300 Real-time PCR System. The relatively lower Ct value was identified than the other methods, which resulted in higher efficiency.
Media:Direct™ Extraction Buffer
| No. | Sample type | 1 | 2 | 3 | 4 | 5 | Average |
|---|---|---|---|---|---|---|---|
| 1 | Nasopharyngeal swab(VTM) | 31.22 | 31.16 | 31.44 | 31.27 | 30.37 | 31.09 |
| 2 | Oropharyngeal swab(VTM) | 29.84 | 31.15 | 30.65 | 30.95 | 30.97 | 30.51 |
Spin Column Method ("A" company)
| No. | Sample type | 1 | 2 | 3 | 4 | 5 | Average |
|---|---|---|---|---|---|---|---|
| 1 | Nasopharyngeal swab(VTM) | 35.30 | 35.43 | 35.38 | 35.32 | 35.71 | 35.43 |
| 2 | Oropharyngeal swab(VTM) | 35.34 | 35.57 | 35.14 | 35.61 | 36.91 | 35.71 |
Magnetic Bead Method ("B" company)
| No. | Sample type | 1 | 2 | 3 | 4 | 5 | Average |
|---|---|---|---|---|---|---|---|
| 1 | Nasopharyngeal swab(VTM) | 33.40 | 33.60 | 33.63 | 33.40 | 33.41 | 33.49 |
| 2 | Oropharyngeal swab(VTM) | 33.02 | 33.28 | 33.20 | 33.01 | 33.01 | 33.10 |
PROTOCOL
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KIT CONTENTS
| Item | Quantity / Volume | Description |
|---|---|---|
| Media:Direct™ Extraction Buffer | 10 μL x 10 tubes | Extraction Buffer |
ORDERING INFORMATION
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